Collaboration with the Zenobi-Wong group published
Adequate vascularization is required for the successful translation of many in vitro engineered tissues. This study presents a novel collagen derivative that harbors multiple recognition peptides for orthogonal enzymatic crosslinking based on sortase A and Factor XIII. Overall, this work demonstrates for the first time the co-engineering of mature micro- and meso-sized blood and lymphatic capillaries using a highly versatile collagen derivative.
Determination of degree of substitution based on 13C-labeled SAT peptide and NMR analysis. a) Structure of (L-Ala-3- 13C)-SAT-4PA used in the labeling of Col-( 13C)SAT. b,c) Standard curve generated from triplicate measurements of (L-Ala-3- 13C)-SAT-4PA (range: 20-100 μM). The internal standard DSS was set to 0 ppm. d) The peak at ~ 1.39 ppm in the 13C-filtered spectrum (Col-( 13C)SAT) was used for the quantification. The respective peak was not visible in the non-modified collagen (Col-SAT).